Biology and Ecology
Allele Frequency Calculator
Allele Frequency Calculator calculate two-allele frequencies from observed AA, Aa, and aa genotype counts and compare observed heterozygosity.
Biology and Ecology
Allele Frequency Calculator
Calculate two-allele frequencies from observed AA, Aa, and aa genotype counts and compare observed heterozygosity.
Formula
Frequency of A = (2 x AA + Aa) / (2 x total individuals); frequency of a = 1 - frequency of A.
About the Allele Frequency Calculator
Calculate two-allele frequencies from observed AA, Aa, and aa genotype counts and compare observed heterozygosity.
How the Allele Frequency Calculator Works
Each homozygote contributes two copies of its allele and every heterozygote contributes one copy of each before division by the total allele count.
Formula
Frequency of A = (2 x AA + Aa) / (2 x total individuals); frequency of a = 1 - frequency of A.
The calculation runs in your browser. Values are validated for required ranges, compatible units, and method-specific restrictions before results are displayed.
Required Inputs
- AA genotype count (required).
- Aa genotype count (required).
- aa genotype count (required).
Results Reported
The result panel reports the final answer and the intermediate quantities needed to check the calculation:
- Allele A frequency
- Allele a frequency
- Total individuals
- Total allele copies
- Observed heterozygosity
- Expected Hardy-Weinberg heterozygosity
Allele Frequency Calculator Example
Select Example Data in the calculator to load this reproducible input set:
| Input | Example value |
|---|---|
| AA genotype count | 50 |
| Aa genotype count | 40 |
| aa genotype count | 10 |
How to Use the Calculator
- Confirm that the calculator title and formula match the quantity you need.
- Enter every required value using the unit shown with its field.
- Select Example Data to inspect a valid input set, or enter your own values and select Calculate.
- Review all reported values and the displayed formula before using the answer.
- Use Copy Result or Download CSV when you need a reusable record.
Accuracy and Limitations
Counts must be mutually exclusive genotypes from the same diploid sample and locus. Missing genotypes, polyploidy, multiple alleles, and genotyping error need different handling.
Keep units consistent, use measurements that represent the actual situation, retain full precision during the calculation, and round only the final answer. Professional decisions may require current official rules, field measurements, laboratory methods, or specialist review.
Frequently Asked Questions
What does the Allele Frequency Calculator calculate?
Calculate two-allele frequencies from observed AA, Aa, and aa genotype counts and compare observed heterozygosity.
Which formula does the Allele Frequency Calculator use?
Frequency of A = (2 x AA + Aa) / (2 x total individuals); frequency of a = 1 - frequency of A. Each homozygote contributes two copies of its allele and every heterozygote contributes one copy of each before division by the total allele count.
What should I check before using the Allele Frequency Calculator result?
Counts must be mutually exclusive genotypes from the same diploid sample and locus. Missing genotypes, polyploidy, multiple alleles, and genotyping error need different handling.